His Tag antibody

Catalog No. ABIN7672605

The Mouse Monoclonal anti-His Tag antibody has been validated for WB and ELISA. It is suitable to detect His Tag in samples from .

Quick Overview for His Tag antibody (ABIN7672605)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 0B1

Product Details anti-His Tag Antibody

Purpose: 6*His, His-Tag antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: A synthetic peptide

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Alternative Name: His-Tag

Target Type: Tag

Background:

Synonyms: 6*His|6*His|His Tag|6histag|6XHis|HHHHHH|HIS|His Tag|HISTIDINE TAGGED

Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.